Plasmid_Backbone

Part:BBa_K390000:Design

Designed by: Cody Tramp   Group: iGEM10_Utah_State   (2010-08-01)


pSB1A3_Int (BamHI, XhoI, NdeI, NheI added)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal NheI site found at 230
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2142
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2136
    Illegal BamHI site found at 1992
    Illegal XhoI site found at 2009
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2136
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2136
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2151
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1175


Design Notes

BamHI, XhoI, NdeI, and NheI sites were mutated in using Stratagene's site-directed mutagenesis kit, the sites were mutated in by changing the nucleotide sequence, without the addition or removal of nucleotides.


Source

pSB1A3 with BamHI, XhoI, NdeI, and NheI sites mutated in outside of the terminator regions.

References